Fig. 3.

Relative OsCKX2 expression levels in selected T1 CX3- and CX5-suppression lines by qRT-PCR. Total RNA was isolated from young stems of wild type (WT) and selected T1 CX3-suppression lines (2–1–1, 2–1–10, 3–5–2, 3–5–7, 8–2–1, 8–2–3) (a) and CX5-suppression lines (1–2–2, 1–2–4, 1–2–5, 3–6–2, 3–6–6, 3–6–7, 5–2–2, 5–2–3) (b). 17S rRNA was used as an internal control for normalization. The expression level of line 2–1–1 and line 3–6–7 were used as a reference in the comparison of CX3- and CX5-suppression lines, respectively. Values presented were mean +/− SD of 3 replicates of cDNA samples