Table 1.
Specific primers used in this study
| Primer name | Sequence (5′ to 3′) | Target gene |
|---|---|---|
| Hygromycin-F | GACCTGATGCAGCTCTCGGAG | hptII |
| Hygromycin-R | TGCTCCATACAAGCCAACCACG | |
| GUS-F | AAAAAACTCGACGGCCTGTGGG | GUS |
| GUS-R | GCATCTTCATGACGACCAAAGC | |
| Ubiquitin pro.-F | CTGATGCATATACAGAGATGC | a CX3 or CX5 |
| Nos ter.-R | TGACAGCTTATCATCGGATC | |
| CKX2-F | GTCCACGACGGCGAGCTCAA | OsCKX2 |
| CKX2-R | TCCATCTTGGCATCTCTCAG | |
| Actin-F | GGTAATGTGTTGGACTCTGG | Actin |
| Actin-R | GCAGTGATCTTCCTTGCTCA | |
| 3′UTR-F | CGGTGACGAGGTGTTCTACAC | OsCKX2 3′ UTR |
| 3′UTR -R | CCAAGATCTCGTCGTTCTGC | |
| rRNA-F | GATAACTCGACGGATCGCACGG | 17S rRNA |
| rRNA-R | GCCTGCTGCCTTCCTTGGATGTG | |
| OsCKX1-F | GCACCCGTGGCTCAACCTG | CKX1 |
| OsCKX1-R | GATGTCGGTGGCCGTCTGG | |
| OsCKX3-F | TTTCTTATGCTGATGTGGGTG | CKX3 |
| OsCKX3-R | GAACATTGCTAATCTGAGGTCC | |
| OsSPL14-F | TGAATTTGACCAAGGAAAAC | SPL14 |
| OsSPL14-R | ATCCAACGTAAAGCTTCTGA | |
| D53-F | CCAAGCAGTTTGAAGCGAC | D53 |
| D53-R | CCGCAAGTTTATCAAAGTCAA | |
| OsTB1-F | CAAGAAATCTCGGCGGCTAG | TB1 |
| OsTB1-R | CGAATTGGCGTAGACGAC |
ashRNA cannot be amplified directly by PCR. Thus, a 867 bp fragment containing partial promoter, shRNA and partial terminator were amplified for detecting the CX3 or CX5 gene