Figure 2.

Ablation of Sox4 induces mesenchymal-to-epithelial transition (MET). (A) Knockdown of Sox4 in 5637 cells as confirmed by immunoblot (β-actin was used as a loading control). (B) Images of control and Sox4-targeted 5637 cells. Knockdown of Sox4 reverses the mesenchymal state of cells back to an epithelial-like state. (C) Increased epithelial marker (E-cadherin) and decreased mesenchymal markers (vimentin and N-Cadherin) in Sox4-knockdown cells as revealed by immunoblot. (D) Increased and plasma membrane-localized E-cadherin in Sox4-knockdown cells as revealed by immunofluorescence microscopy (scale bar=20µm). (E) Knockdown of Sox4 in 5637 cells induced dramatically repression of ZEB2 expression as revealed by real time RT-PCR. (** p<0.01)