Skip to main content
. 2015 Nov 1;11(12):1363–1375. doi: 10.7150/ijbs.13240

Figure 5.

Figure 5

Gene expression profiling reveals Sox4 regulated genes. (A) GSEA enrichment plot shows that focal adhesion components are highly enriched and cell cycle regulated genes significantly reduced in response to siSox4 in RT-112 cells. (B) Heat map of hierarchically clustered genes in RT-112 cells expressing scrambled control (sicontrol) or siSox4 (siSox4). Expression levels are from duplicate samples. Significantly up-regulated (red) and down-regulated (blue) GO terms and enrichment p values by DAVID analysis are shown. (C) Real-time RT-PCR of Sox4, CCND1, CDK1, FGFR1, FGFR3, MYB, MYC, TUBA1A, TUBA1C, TUBB3 and TUBB4B mRNA followed by treatment with siControl or siSox4 in the RT-112 cells. GAPDH and β-actin were used as internal controls. (D) Real-time RT-PCR of CRB3, FERMT2, NEDD9, PARD6B, SHANK2, TJP1, TJP3 and S100A8 mRNA followed by treatment with siControl or siSox4 in the RT-112 cells. GAPDH and β-actin were used as internal controls.