Figure 1.

Distribution of cysteine-rich 61 (Cyr61) proteins in the endometrium in women with and without PCOS. Human endometrial tissues were fixed in formalin and embedded in paraffin, and immunohistochemistry was performed. It was reported that the expression of Cyr61, a cysteine-rich secreted heparin-binding protein, was significantly increased in the endometrium of PCOS patients with hyperplasia compared to age-matched, non-PCOS patients with normal menstrual cycles, and it appears to be a useful tissue biomarker for the detection of endometrial hyperplasia. Therefore, we used Cyr61 protein expression to verify that the endometrial samples had come from non-PCOS and PCOS patients. Representative images from three independent experiments are shown. The histology of hematoxylin/eosin-stained human endometrial biopsy samples is indicated. GE, glandular epithelial cells; Str, stromal cells.