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. 2015 Dec 8;3:197. doi: 10.3389/fbioe.2015.00197

Figure 5.

Figure 5

Confirmation of specificity of the Zera®M2e mice sera against the fused M2e plant-produced protein. Equal volume of plant-produced 5xM2e_tHA proteins were loaded in each lane. The membranes were cut in the middle and each half was probed either with the commercial M2 antibody or mice serum from mice immunized with plant-produced Zera®M2e PB. Lanes 1 and 5 contain Agrobacterium-infiltrated crude plant extract (negative control), Lanes 2 and 4 contain the 5xM2e_tHA plant crude extract, and Lane 3 contains PageRuler™ Prestained protein ladder (Fermentas). Lanes 1 and 2 were detected with commercial M2 (14C2) primary antibody (1:5000). Lanes 4 and 5 were detected with mice sera from mice immunized with plant-produced Zera®M2e PB (1:100).