Table 1.
List of strains and plasmids used in this study.
| Strain/plasmid | Description | Source |
|---|---|---|
| S. pneumoniae | ||
| D39 | Serotype 2 strain, cps 2 | Laboratory of P. Hermans |
| SS200 | D39 ΔadcR; EryR | Shafeeq et al., 2011a |
| IM404 | D39 ΔbgaA::PczcD-lacZ; TetR | Manzoor et al., 2015a |
| IM501 | D39 ΔbgaA::PadcR-lacZ; TetR | This study |
| IM502 | D39 ΔbgaA::PadcAII-lacZ; TetR | This study |
| IM503 | D39 ΔbgaA::PphtA-lacZ; TetR | This study |
| IM504 | D39 ΔbgaA::PphtB-lacZ; TetR | This study |
| IM505 | D39 ΔbgaA::PphtE-lacZ; TetR | This study |
| IM551 | SS200 ΔbgaA::PadcR-lacZ; TetR | This study |
| IM552 | SS200 ΔbgaA::PadcAII-lacZ; TetR | This study |
| IM553 | SS200 ΔbgaA::PphtA-lacZ; TetR | This study |
| IM554 | SS200 ΔbgaA::PphtB-lacZ; TetR | This study |
| IM555 | SS200 ΔbgaA::PphtE-lacZ; TetR | This study |
| E. coli | ||
| EC1000 | KmR; MC1000 derivative carrying a single copy of the pWV1 repA gene in glgB | Laboratory collection |
| Plasmids | ||
| pPP2 | AmpR TetR; promoterless lacZ For replacement of bgaA with promoter lacZ-fusion. Derivative of pPP1 | Halfmann et al., 2007 |
| pIM501 | pPP2 PadcR-lacZ | This study |
| pIM502 | pPP2 PadcAII-lacZ | This study |
| pIM503 | pPP2 PphtA-lacZ | This study |
| pIM504 | pPP2 PphtB-lacZ | This study |
| pIM505 | pPP2 PphtE-lacZ | This study |
| SS107 | pNZ8048 carrying strep-tagged AdcR downstream of PnisA | Shafeeq et al., 2011a |