Skip to main content
. 2015 Sep 14;26(1):13–18. doi: 10.1093/glycob/cwv076

Fig. 2.

Fig. 2.

OGT modifies Nod2. (A) Cell lysates were immunopurified with Myc antibody and blotted with Myc antibody; Control cells do not express Myc-Nod2. Thiamet-G inhibits OGA, elevating O-GlcNAcylation. (B) Cell lysates were immunopurified with Nod2 and blotted using CTD110.6. (C) Alternatively, the CTD110.6 was pre-incubated with free GlcNAc. (D) Co-IP were performed using 1 µl of anti-O-GlcNAc (RL2 or CTD110.6) antibody per 200 µL of lysate (HEK293T/Nod2Myc cells) and probed for Myc using rabbit anti-Myc antibody (1:1000), anti-RL2 antibody (1:2000) and anti-CTD110.6 (1:1000).