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. 2015 Dec;149(7):1896–1909.e14. doi: 10.1053/j.gastro.2015.08.053

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Contribution of hepatic phagocytes to clearance of pathogenic material. (A) Immunohistochemistry of hepatic macrophages (F4/80 = green) after isolation by adherence and administration of E coli bioparticles (pHrodo) (red). (B) Relative number and percentage of resident macrophages phagocytosing E coli bioparticles (pHrodo, ThermoFisher Scientific, Waltham, MA). Representative density plots of phagocytic cells in the resident macrophage population. (C) Relative number of infiltrating macrophages phagocytosing E coli bioparticles compared with the mean of the control group with the percentage of cells phagocytosing E coli particles according to Ly6C expression status. (D) Pie charts illustrating the proportion of phagocytic cells in the liver with a bar chart showing the absolute cell number comparison for resident and infiltrating macrophages, neutrophils, and eosinophils. Eos, eosinophils; Neuts, neutrophils. *P < .05, ** P < .01, *** P < .001, **** P < .0001.