Table 1.
Ingredient composition and nutrient content of different broiler chicken diets used for in vitro digestion with or without inclusion of Bacillus-DFM candidate spore on as-is basisa.
| Item | Corn-based diet | Wheat-based diet | Barley-based diet | Rye-based diet | Oat-based diet |
|---|---|---|---|---|---|
| INGREDIENTS (g/kg) | |||||
| Corn (80 g/kg CP) | 619.6 | – | – | – | – |
| Wheat (135 g/kg CP) | – | 711.0 | – | – | – |
| Barley (113 g/kg CP) | – | – | 654.3 | – | – |
| Rye (126 g/kg CP) | – | – | – | 622.6 | – |
| Oats (98 g/kg CP) | – | – | – | – | 638.0 |
| Soybean meal (475 g/kg CP) | 298.2 | 203.9 | 241.9 | 264.6 | 260.0 |
| Poultry oil | 39.1 | 42.8 | 65.0 | 70.0 | 70.0 |
| Dicalcium phosphate | 16.9 | 17.1 | 17.0 | 16.6 | 16.4 |
| Calcium carbonate | 10.6 | 8.5 | 8.2 | 10.4 | 10.0 |
| Salt | 3.8 | 3.0 | 3.0 | 5.7 | 2.0 |
| DL-Methionine | 3.3 | 2.5 | 3.0 | 3.0 | 3.2 |
| L-Lysine HCL | 2.8 | 4.6 | 2.0 | 2.0 | 1.6 |
| Threonine | 1.2 | 2.1 | 1.1 | 0.6 | 0.6 |
| Choline chloride 60% | 2.0 | 2.0 | 2.0 | 2.0 | 2.0 |
| Vitamin premixb | 1.0 | 1.0 | 1.0 | 1.0 | 1.0 |
| Mineral premixc | 1.0 | 1.0 | 1.0 | 1.0 | 1.0 |
| Antioxidantd | 0.5 | 0.5 | 0.5 | 0.5 | 0.5 |
| CALCULATED ANALYSIS | |||||
| Metabolizable energy (MJ/kg) | 13.0 | 13.0 | 12.3 | 12.2 | 11.9 |
| Crude protein (g/kg) | 195.0 | 200.0 | 190.0 | 205.0 | 186.4 |
aInclusion of 108 spore/g of feed mixed with calcium carbonate.
bVitamin premix supplied per kilogram of diet: retinol, 6 mg; cholecalciferol, 150 μg; DL-α-tocopherol, 67.5 mg; menadione, 9 mg; thiamine, 3 mg; riboflavin, 12 mg; pantothenic acid, 18 mg; niacin, 60 mg; pyridoxine, 5 mg; folic acid, 2 mg; biotin, 0.3 mg; cyanocobalamin, 0.4 mg.
cMineral premix supplied per kg of diet: Mn, 120 mg; Zn, 100 mg; Fe, 120 mg; copper, 10–15 mg; iodine, 0.7 mg; selenium, 0.2 mg; and cobalt, 0.2 mg.
dEthoxyquin.