Table 2.
Relative enzyme activity values (REA) and Clostridium perfringens zone of inhibition produced by different Bacillus spp. strains present in the Bacillus-DFM candidate treatment.
| Measurements | AM1002 | AM0938 | JD17 |
|---|---|---|---|
| CELLULASE ACTIVITY AT 24 h | |||
| Colony size (mm) | 5.7 ± 0.33a | 6.0 ± 0.58a | 6.3 ± 0.33a |
| Zone of clearance (mm) | 35.2 ± 1.76a | 30.7 ± 0.67a,b | 29.3 ± 2.19b |
| REAc | 6.2 ± 0.12a | 5.1 ± 0.49a,b | 4.7 ± 0.29b |
| XYLANASE ACTIVITY AT 24 h | |||
| Colony size (mm) | 5.0 ± 0.58b | 6.7 ± 0.33a,b | 7.3 ± 0.67a |
| Zone of clearance (mm) | 31.7 ± 0.88a | 32.0 ± 1.15a | 29.0 ± 1.53a |
| REAc | 6.3 ± 0.87a | 4.9 ± 0.43a,b | 4.0 ± 0.15b |
| C. perfringens AT 24 h | |||
| Zone of inhibition (mm)d | 12.3 ± 1.45a | 14.0 ± 1.00a | 8.0 ± 1.15b |
a,bSuperscripts within a row with no common superscript differ significantly P < 0.05.
cRelative enzyme activity values (REA) reflect the capacity to produce cellulase and xylanase enzymes by Bacillus spp. REA was calculated dividing the diameter of area of clearance by the diameter of the Bacillus colony. Based on REA test, organism can be categorized into three groups showing excellent (REA > 5.0), good (REA > 2.0–5.0), or poor (REA < 2.0) relative enzyme activity. All Bacillus spp. isolates were tested by triplicate. Data expressed as mean ± SE.
dRepresents the diameter of the zone of inhibition observed at 24 h of incubation without the diameter of the bacterial colony. All Bacillus spp. isolates were tested by triplicate. Data expressed as mean ± SE.