Table 1. Efficiency of single cell-derived colony formation from a HeLa cell in soft agar culture with or without hMSCs (12,500 and 62,500 cells).
HeLa | HeLa/hMSCs (12,500) | HeLa/hMSCs (62,500) | hMSCs (12,500) | hMSCs (62,500) | |
---|---|---|---|---|---|
Efficiency of colony formation (%) | 44.7 ± 2.2a | 68.8 ± 9.2# | 70.0 ± 3.4# | Not detected | Not detected |
aThe ratio of the total number of colonies to the number of transformed cells dispensed in a 96-well plate were defined as the colony-forming efficiency of transformed cells in soft agar culture. Values are means ± SD of three experiments. Statistical significance was determined using one-way ANOVA and Dunnett’s multiple comparisons test (#P < 0.05, compared with the HeLa cell alone group). hMSCs, human mesenchymal stem cells.