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. 2015 Nov 16;2:55. doi: 10.3389/fvets.2015.00055

Figure 9.

Figure 9

Induced pluripotent stem cells remain in cell clusters in 3D gels. (A) iPSCs in 3D constructs at 28 days remain in cell clusters. Some clusters demonstrate fibroblastic outgrowths, but these are not specifically aligned to the direction of force (arrow). (Bi) In contrast, H&E-stained sections of ESC 3D constructs after 14 days demonstrate that the majority of cells are at the edges of the gels and are evenly distributed. A higher magnification image is shown in (Bii). (Ci) Occasional clusters of cells are still present in some ESC 3D constructs after 14 days. A higher magnification image is shown in (Cii). Scale bar = 100 μm.