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. 2015 Dec 8;5:17829. doi: 10.1038/srep17829

Figure 2. Autophagy in pronucleus stage formation.

Figure 2

(a) Coexpression of LC3 and p62 in the meiosis II (M II), pronucleus stage of ICSI, NT (CNT), rapamycin treated NT (RNT), and pp242 treated NT(PNT). Green, LC3 labelled protein; Red, p62 labelled protein; Blue, chromatin; Merge, LC3 (green), p62 (red) and chromatin (blue) merged images. Note that the colocalisation of LC3 and p62 is indicated by an arrow in the enlarged panel, which is indicated by the white box. Scale bar, 20 μm. (b) Quantification of LC3 and p62 merged dots in oocytes and embryos. Each value represents the mean ± s.e.m. *p < 0.05; **p < 0.01; ns, not significant.