Figure 4. Activation of autophagy greatly improves development of SCNT embryos.

10 nM of rapamycin or 100 nM of pp242 was added into activation medium for 6 h during the activation of NT embryos. (a) The embryonic stages at 22 h, 46 h, 72 h and 96 h after activation were calculated as mean ± SD. *p < 0.05. (b) Representative bright-field photographs of embryos at blastocyst stage are shown. Scale bar, 100 μm.