Figure 6. Degradation of selective maternal mRNA after fertilization or activation.

mRNA was isolated from embryos 3 h, 6 h, 9 h and 22 h after sperm injection or activation, Reverse-transcribed using oligo(dT) primer, and subjected to qPCR to examine the changes in the relative amount of c-mos, Plat, Gdf9 and H1oo mRNAs. The values of the M II oocytes were set as onefold. Three independent experiments were performed and averaged values are shown. *p < 0.05; **p < 0.01.