Table 2. Summary of the sequencing and assembly.
LibrariesZ | Control (1) | Control (2) | Control (3) | EOD-FR (1) | EOD-FR (2) | EOD-FR (3) | All |
---|---|---|---|---|---|---|---|
Total reads | 56,397,212 | 66,135,068 | 77,080,190 | 62,863,124 | 62,960,474 | 71,124,618 | |
Total nucleotides | 5,639,721,200 | 6,613,506,800 | 7,708,019,000 | 6,286,312,400 | 6,296,047,400 | 7,112,461,800 | |
GC percentage | 44.16 | 44.16 | 44.29 | 44.07 | 43.92 | 44.06 | |
Contig | 218,897 | 244,846 | 245,125 | 235,202 | 230,893 | 241,331 | |
Mean Length of contig (nt) | 303 | 302 | 304 | 304 | 305 | 304 | |
Contig N50 | 483 | 471 | 479 | 483 | 486 | 478 | |
Unigene | 91,162 | 105,911 | 105,737 | 100,700 | 100,155 | 103,437 | 161,051 |
Mean Length of unigene (nt) | 1,013 | 1,042 | 1,025 | 1,042 | 1,033 | 1,034 | 1,340 |
Unigene N50 | 1,784 | 1,849 | 1,827 | 1,841 | 1,825 | 1,831 | 2,242 |
Distinct Clusters | 44,242 | 50,677 | 50,756 | 48,511 | 48,605 | 49,835 | 84,563 |
Distinct Singletons | 46,920 | 55,234 | 54,981 | 52,189 | 51,550 | 53,602 | 76,488 |
Z6 libraries as biological replicates constructed from the floral buds of each sample number. Number of replicates shown in brackets.