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. 2015 Dec 8;5:17916. doi: 10.1038/srep17916

Figure 2. Runx3 is strongly induced by dsRNA poly(I:C) in airway epithelial cells.

Figure 2

(a,b) SAECs (a) and BEAS-2B (b) cells were treated 24 h with control PBS (control), low molecular weight poly(I:C) (PIC-L, 10 μg/ml), high molecular weight poly(I:C) (PIC-H, 10 μg/ml), R837 (10 μg/ml), CL075 (10 μg/ml), TGFβ (10 ng/ml), TNFα (6 ng/ml), CpG (2 μg/ml), LPS (1 μg/ml), or Pam3CSK4 (Pam3, 10 μg/ml) as indicated. (c) BEAS-2B cells were treated 24 h with different doses of a high molecular weight poly(I:C) (PIC-H) (left panels) or with the poly(I:C) (5 μg/ml) for 0-24 h (right panels). (d) BEAS-2B cells were treated 24 h with different doses of a high molecular weight poly(I:C) complexed with (+) or without (–) lipofectamine-2000. Cell lysates at equal protein amounts from (a–d) were subjected to Western blot analysis with Runx3 or actin antibodies. Results shown are representative Western blots of three independent experiments. (e) BEAS-2B cells were treated with control PBS or poly(I:C) (2 μg/ml) for 20 h, fixed and immunostained with Runx3 monoclonal antibody or stained with DAPI as indicated. Nuclear localization of Runx3 was visualized and photographed by fluorescence microscopy. Results represent the findings of two independent experiments.