Table 2.
| Sample ID | Concentration (IU/rxn) | Quantitative RFU values (at λ 540) | Fluorogenic Detection | Electrophoretic Detection |
|---|---|---|---|---|
| A. Fluorimetric evaluation of sensitivity of multiplex assay with DNA | ||||
| 1. NTC | NA | 710 | − | − |
| 2. CMV | NA | 883 | − | − |
| 3. HBV-DNA | 104 | 3015 | + | + |
| 4. HBV-DNA | 103 | 2915 | + | + |
| 5. HBV-DNA | 500 | 2201 | + | + |
| 6. HBV-DNA | 102 | 1744 | + | + |
| 7. HBV-DNA | 50 | 1685 | + | + |
| 8. HBV-DNA | 25 | 1414 | + | − |
| Sample ID | Concentration (IU/rxn) | Quantitative RFU values (at λ 613) | Fluorogenic Detection | Electrophoretic Detection |
| 1. NTC | NA | 21 | − | − |
| 2. NC | NA | 23 | − | − |
| 3. HCV-RNA | 103 | 123 | + | + |
| 4. HCV-RNA | 102 | 70 | + | + |
| 5. HCV-RNA | 10 | 45 | + | − |
Spectrofluorometric evaluation of multiplex assay sensitivity. Abbreviations: NTC, no template control; CMV, cytomegalovirus; NC, negative control; HBV, hepatitis B virus; HCV, hepatitis C virus; RFU, relative fluorescence unit; IU/rxn, international units per reaction; NA, not applicable; Note: TET/BHQ1 used for DNA and Texas-Red/BHQ2 used for RNA detection.