Table 1. Strains and plasmids for alterations.
| Strain | Genotype | Reference |
|---|---|---|
| Strains | ||
| Escherichia coli K-12 MG1655 | F- λ- ilvG- rfb-50 rph-1 | A. Feist1 |
| Escherichia coli W | F-, soil isolate, harbors pRK1 and pRK2 | DSMZ 1116 |
| SIJ488 | E. coli K-12 MG1655Tn7::para-exo-beta-gam; prha-FLP; xylSpm-IsceI | This study |
| Plasmid | Description | Reference |
| Plasmids for deletions | ||
| pSIJ8 | pkd46, rhaRS-prha-FLP, amp | This study |
| pSIJ214 | pEMG, MG1655-tn7-para-exo-beta-gam; prha-flp; xylSpm-I-SceI, km | This study |
| Plasmids for frt flanked deletion cassettes | ||
| pkd3 | ApR, FRT-cmR-FRT, oriR6K | 37 |
| pkd4 | ApR, FRT-KmR-FRT, oriR6K | 37 |
| pSIJ196 | pEMG amp, frt::spec::frt | This study |
| pSIJ197 | pEMG amp, frt::gm::frt | This study |
1This strain was generously donated by Dr. Adam Feist, University of California, San Diego.