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. Author manuscript; available in PMC: 2017 Jan 1.
Published in final edited form as: J Immunother. 2016 Jan;39(1):15–26. doi: 10.1097/CJI.0000000000000103

Table 2.

Design of 11-color FACS to quantify surface markers with significant T cell functions.

Laser Dye Stain Index Surface marker Significant function
Violet laser _405nm BD Horizon V450 65 CD25 T cell activation and proliferation
BD Horizon V500 27 CD8 T cell marker
Blue laser_488nm AF488 43 CD185(CXCL5) Activation of B cells
PerCP 99 Tim-3 Tim3+PD-1+ defines exhausted T cells
Green laser_561nm PE 305 CD62L L-selectin marker
PE-Cy5 CD154(CD40L) Activation of dendritic cells
PE-Cy7 122 CD197(CCR7) Lymphatic tissue homing marker
PE-Cy5.5 CD4 T helper cell marker
Red Laser_640nm APC 184 CD279 (PD-1) Tim3+PD-1+ defines exhausted T cells
AF700 64 CD27 Antigen experiencing
APC-H7 25 CD69 T cell Proliferation