Table 5.

Percentage and total number of various CD4⁺ T cell subsets within the day-14 MDLN co-cultures with medium, A375, U87 and MDA-MB-231. n = 4.

		Day-14 MDLN cells + medium	Day-14 MDLN cells + A375	Day-14 MDLN cells + U87	Day-14 MDLN cells + MDA-MB-231
T cell number/million		1580 ± 120	1590 ± 210	1455 ± 230	1585 ± 180
CD4⁺	Cell percentage	63.4% ± 3.3%	82.2% ± 4.8% ^*	59.2% ± 5.1%	63.4% ± 2.3%
CD4⁺	Cell number/million	1002 ± 76	1306 ± 60 ^*	861 ± 110	1004 ± 130
CD4⁺CD62L⁺CCR7⁺	Cell percentage	50.7% ± 1.8%	78.1% ± 2.1% ^*	50.3% ± 1.6%	52.0% ± 1.7%
CD4⁺CD62L⁺CCR7⁺	Cell number/million	801 ± 28	1241 ± 34 ^*	732 ± 23	522 ± 25
CD4⁺CD40L⁺	Cell percentage	2.3% ± 3.4%	26.7% ± 7.8% ^*	9.9% ± 7.1%	10.3% ± 5.5%
CD4⁺CD40L⁺	Cell number/million	36 ± 54	425 ± 124 ^*	144 ± 103	163 ± 87
CD4⁺CXCR5⁺	Cell percentage	6.7% ± 5.1%	51.3% ± 9.7% ^*	7.6% ± 6.3%	9.4% + 5.5%
CD4⁺CXCR5⁺	Cell number/million	106 ± 81	816 ± 154 ^*	111 ± 92	149 ± 87
CD4⁺CD27⁻	Cell percentage	15.3% ± 2.8%	46.4% ± 3.6% ^*	30.6% ± 2.2%	23.2% ± 7.1%
CD4⁺CD27⁻	Cell number/million	241 ± 44	483 ± 57 ^*	271 ± 32	368 ± 113
CD4⁺CD62L⁺CCR7⁺CD27⁻	Cell percentage	12.5% ± 2.1%	43.1% ± 3.9% ^*	28.2% ± 15.6%	15.5% ± 4.3%
CD4⁺CD62L⁺CCR7⁺CD27⁻	Cell number/million	197 ± 33	685 ± 62 ^*	453 ± 240	245 ± 68
CD4⁺CD62L⁺CCR7⁺CD27⁺	Cell percentage	39.9% ± 5.6%	26.7% ± 7.5%	25.4% ± 13.7%	37.2% ± 5.1%
CD4⁺CD62L⁺CCR7⁺CD27⁺	Cell number/million	630 ± 88	425 ± 120	370 ± 188	590 ± 81
CD4⁺CD62L⁺CCR7⁺CD27⁻CXCR5⁺	Cell percentage	Not detected	36.6% ± 3.3% ^*	Not detected	0.9% ± 0.4%
CD4⁺CD62L⁺CCR7⁺CD27⁻CXCR5⁺	Cell number/million	Not detected	582 ± 52.1 ^*	Not detected	14 ± 6.2
CD4⁺CD62L⁺CCR7⁺CD27⁻CD40L⁺	Cell percentage	Not detected	23.4% ± 3.5% ^*	10.2% ± 5.2%	Not detected
CD4⁺CD62L⁺CCR7⁺CD27⁻CD40L⁺	Cell number/million	Not detected	373 ± 55.3 ^*	162 ± 82.4	Not detected
CD4⁺CD62L⁺CCR7⁺CD27⁻IL-2⁺	Cell percentage	1.1% ± 5.7%	18.2% ± 10.2% ^*	14.3% ± 3.3%	2.7% ± 0.9%
CD4⁺CD62L⁺CCR7⁺CD27⁻IL-2⁺	Cell number/million	17 ± 90	289 ± 162 ^*	208 ± 48	43 ± 14
CD4⁺CD62L⁺CCR7⁺CD27⁻TNF-α⁺	Cell percentage	2.1% ± 1.1%	12.3% ± 2.6% ^*	9.1% ± 1.5%	2.6% ± 0.9%
CD4⁺CD62L⁺CCR7⁺CD27⁻TNF-α⁺	Cell number/million	33 ± 17	196 ± 41 ^*	132 ± 22	41 ± 14
CD4⁺CD62L⁺CCR7⁺CD27⁻IL-4⁺	Cell percentage	Not detected
CD4⁺CD62L⁺CCR7⁺CD27⁻IL-4⁺	Cell number/million	Not detected
CD4⁺CD62L⁺CCR7⁺CD27⁻IFN-γ⁺	Cell percentage	Not detected
CD4⁺CD62L⁺CCR7⁺CD27⁻IFN-γ⁺	Cell number/million	Not detected
CD4⁺CD62L⁺CCR7⁺CD27⁺IL-2⁺	Cell percentage	41.3% ± 5.3%	37.2% ± 4.1%	35.2% ± 3.7%	34.3% ± 6.5%
CD4⁺CD62L⁺CCR7⁺CD27⁺IL-2⁺	Cell number/million	653 ± 84	591 ± 65	512 ± 54	541 ± 103
CD4⁺CD62L⁺CCR7⁺CD27⁺TNF-α⁺	Cell percentage	32.9% ± 6.1%	41.2% ± 1.1%	37.8% ± 3.3%	39.4% ± 2.5%
CD4⁺CD62L⁺CCR7⁺CD27⁺TNF-α⁺	Cell number/million	520 ± 96	655 ± 16	549 ± 48	624 ± 40
CD4⁺CD62L⁺CCR7⁺CD27⁺IL-4⁺	Cell percentage	6.6% ± 2.1%	8.6% ± 4.7%	9.5% ± 4.3%	9.9% ± 2.1%
CD4⁺CD62L⁺CCR7⁺CD27⁺IL-4⁺	Cell number/million	104 ± 33	136 ± 75	138 ± 63	157 ± 35
CD4⁺CD62L⁺CCR7⁺CD27⁺IFN-γ⁺	Cell percentage	Not detected
CD4⁺CD62L⁺CCR7⁺CD27⁺IFN-γ⁺	Cell number/million	Not detected

significantly different from the non-stimulated control (medium) and the controls for allo-responses (U87 and MDA-MB-231).