Table 3. Species and cluster of leptospiral DNA sequences recovered from animals in 2009 and 2013, Portoviejo, Ecuador*.
Location, year, animal, no analyzed samples |
Leptospira spp.–positive samples |
No. (%) spurious PCR products† | ||||
---|---|---|---|---|---|---|
Pathogenic cluster |
Intermediate cluster |
|||||
No. (%) | Species | No. (%) | Species | |||
Portoviejo, 2009‡ | ||||||
Cattle, n = 7 | 3 (43) | L. borgpetersenii | 4 (57) | L. inadai | 0 | |
Rats, n = 6 | 1 (17) | L. borgpetersenii | 5 (83) | L. inadai | 0 | |
Dogs, n = 3 | 0 | – | 3 (100) | L. inadai | 0 | |
Pigs, n = 3 |
1 (33) |
L. borgpetersenii
|
|
2 (67) |
L. inadai
|
0 |
Portoviejo, 2013§ | ||||||
Cattle, n = 26 | 5 (19) | L. borgpetersenii | 1 (4) | L.wolffii | 3 (27) | |
1 (4) | L. kirschneri | – | – | |||
Rats, n = 60 | 3 (5) | L. borgpeterseni | 1 (1.7) | L.wolffii | 3 (21) | |
2 (3.3) | L. kirschner¶ | – | – | |||
Pigs, n = 30 |
2 (6.7) |
L. borgpetersenii
|
|
1 (3.3) |
L.wolffii
|
5 (50) |
*–, not applicable/no value. †Percentage of amplicons (obtained from samples of each animal species) which showed expected size but the DNA sequences were different from Leptospira spp. ‡Dry season. §Rainy season. ¶One amplicon also showed the same degree of identity to L. interrogans. |