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Visceralization of Leishmania infantum from US foxhounds, transmitted by sandflies into hamsters. Leishmania spp.–specific quantitative PCR was performed, and parasite load was calculated from a standard curve. Horizontal bars indicate mean values for 3 experiments run in duplicate. Statistical significance was determined by 1-way analysis of variance with Bonferroni posttest between 6 naive and 15 infected groups, by tissue type. Error bars indicate ± SEM. *p<0.05.
