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. 2015 Dec;21(12):2244–2246. doi: 10.3201/eid2112.141249

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A) Nucleotide sequence of vaccinia virus (VACV) hemagglutinin gene and homologous sequences of several orthopoxviruses, Brazil. Dots indicate sequence identity; dashes indicate deletions. VARV, variola virus; MPXV, monkeypox virus; CPXV, cowpox virus. B) Consensus phylogenetic tree based on nucleotide sequences of orthopoxvirus hemagglutinin genes. Tree was constructed with hemagglutinin gene sequences by using the neighbor-joining method with 1,000 bootstrap replicates and the Tamura 3-parameter model in MEGA4 (http://www.megasoftware.net/). Strains had the deletion region conserved and were grouped with other VACV (group 2) isolated in Brazil. Numbers along branches are bootstrap values. Scale bar indicates nucleotide substitutions per site.