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. 2015 Dec 8;14:205. doi: 10.1186/s12943-015-0477-z

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© Kreuz et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — PIM1 and c-MYC bind to the GNL3 promoter. ChIP was carried out using chromatin from Raji cells and antibodies against PIM1 (a), c-MYC (b), Pol II CTD (c) or H3K9acS10p (d). Eluted gDNA was then subjected to qPCR using primer pairs in different regions of the GNL3 gene. a qPCR results were normalised to input and the GNL3 -3.5 kb region. Means and SD of six independent experiments are shown. b Results are shown normalised to input and the GNL3 -3.5 kb region. Means and sd of five independent experiments are shown for Raji cells, whereas one experiment was carried out in OCI-Ly10 cells. c Results are again normalised to input and the GNL3 -3.5 kb region. Means and SD of two independent experiments are shown for Raji cells, one experiment was done in OCI-Ly10 cells. d Results are normalised to H3 and the GNL3 -3.5 kb region. The means and SD of three independent experiments are shown