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. 2015 Dec 8;6:1368. doi: 10.3389/fmicb.2015.01368

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Copyright © 2015 Chouhan, Islamuddin, Want, Ozbak, Hemeg, Sahal and Afrin.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Visualization of PS exposure. (A) Promastigotes (2 × 10⁶ ml^-1) after treatment with test fractions and compounds (500 μg ml^-1) for 72 h at 22°C were co-stained with Annexin-V-FLUOS and PI. The bar graph depicts percentages of live, apoptotic, secondary apoptotic and necrotic cells for different test samples. ^∗∗∗P < 0.001 and ^∗∗P < 0.01 with respect to parasite control. (B) FL-1 (x-axis) vs. FL-2 (y-axis) dot plots depicting live (both annexin and PI negative, lower left quadrant), necrotic (PI positive, upper left quadrant), apoptotic (annexin positive, lower right quadrant) and secondary necrotic (both annexin and PI postive) Leishmania parasites.