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. 2015 Dec 8;6:1368. doi: 10.3389/fmicb.2015.01368

FIGURE 4.

FIGURE 4

In vivo therapeutic potential of P. nigrum bioactive fractions. (A) Estimation of parasite burden in untreated and bioactive fractions treated mice. VC = vehicle control (0.5% DMSO in PBS), INF = Infection control, AmB = AmB at 5 mg/kg bw, PNH100 = P. nigrum hexane fraction at 100 mg/kg bw, PNH200 = P. nigrum hexane fraction at 200 mg/kg bw, PNE100 = P. nigrum ethanolic fraction at 100 mg/kg bw, PNE200 = P. nigrum ethanolic fraction at 200 mg/kg bw and PIP200 = Piperine at 200 mg/kg bw. L. donovani infected BALB/c mice were either untreated or subjected to different treatments followed by estimation of hepatic and splenic parasite burden as described in methods. ∗∗∗P < 0.001, ∗∗P < 0.01, and ∗P < 0.05 in relation to INF group. (B) Differences in liver and spleen weights amongst various experimental groups. The dotted line represents average weight of respective organs in normal mice. #P < 0.001 in comparison to naive mice, for rest ∗∗∗P < 0.001, ∗∗P < 0.01, ∗P < 0.05, and ns = non-significant with respect to INF.