FIGURE 7.

Determination of changes in splenic T cell populations. (A) To distinguish CD4⁺ and CD8⁺ T cell population, co-staining with anti-CD4-PE and anti-CD8-FITC antibodies was performed as described in the Section “Materials and Methods”. Bar graphs display significant and non-significant changes in CD4⁺ and CD8⁺ T cell numbers post-treatment with bioactive fractions. ^∗∗∗P < 0.001, ^∗∗P < 0.01, ^∗P < 0.05, and ns = non-significant with respect to INF. (B) Dot plots also depicting the changes in splenic CD4⁺ and CD8⁺ T cell numbers after treatment with P. nigrum bioactive fractions.