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. 2015 Dec 8;6:1368. doi: 10.3389/fmicb.2015.01368

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Copyright © 2015 Chouhan, Islamuddin, Want, Ozbak, Hemeg, Sahal and Afrin.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Analysis of Th1 subset potentiation. (A) Estimation of Th1 and Th2 cytokine levels. INF-γ, TNF-α, IL-2, IL-4, and IL-10 were quantified in culture supernatants of splenocytes post 48 h of SLA stimulation as described in the Section “Materials and Methods”. ^#P < 0.001 in comparison to normal group, ^∗∗∗P < 0.001, ^∗∗P < 0.01, and ^∗P < 0.05 with respect to INF. (B) IgG isotyping in sera of untreated and differently treated BALB/c mice. Leishmanial antigen specific IgG2a and IgG1 isotypes were quantified in BALB/c mice serum samples by ELISA. #,indicates significant elevation in IgG1 levels in INF and VC group in comparison to naïve mice. ^∗∗∗P < 0.001, and ^∗∗P < 0.01 with respect to INF.