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. 2015 Dec 8;6:1368. doi: 10.3389/fmicb.2015.01368

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Copyright © 2015 Chouhan, Islamuddin, Want, Ozbak, Hemeg, Sahal and Afrin.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Assessment of immunomodulatory effect of bioactive fractions on murine macrophages. (A) Estimation of CD80 and CD86 expression. Peritoneal macrophages from different treatment groups were exposed to surface staining with antimouse-CD80-APC and antimouse-CD86-PeCy7 along with appropriate controls. Individual percentages of CD80 and CD86 positive cells for each group were deduced with the help of BD FACS DIVA software. ^∗∗∗P < 0.001, ^∗∗P < 0.01, ^∗P < 0.05, and ns = non-significant with respect to INF. (B) Assessment of in vivo NO production. Culture supernatants from splenocytes of all the experimental groups were isolated and incubated with equal volume of griess reagent for NO estimation as described in methodology section. ^∗∗∗P < 0.001 and ns = non-significant with respect to INF.