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. Author manuscript; available in PMC: 2016 Oct 20.
Published in final edited form as: Immunity. 2015 Oct 20;43(4):715–726. doi: 10.1016/j.immuni.2015.10.005

Figure 1. C-terminal CARD9 Truncations Impair Depleted Zymosan- and TDM-induced Cytokine Production.

Figure 1

(A) Schematic of CARD9 variants used. All indicated variants were found in a human immune cDNA panel with the exception of CARD9 416-536, which was designed for experimental use. CC: coiled-coil domain. (B–D) Card9−/− murine BMDCs transduced with indicated CARD9 variants were stimulated with either depleted zymosan, TDM or LPS and cytokine levels were assessed by ELISA. Data were obtained from three independent experiments performed in duplicate (n = 3). (E) Expression of CARD9 variants in total lysates from (B–D) as detected by Western blot. Bars represent means ± s.d. *P < 0.05, **P < 0.01. Comparisons in (B–D) are relative to stimulated CARD9 WT. See also Figure S1.