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. 2015 Nov 16;112(48):E6673–E6682. doi: 10.1073/pnas.1516729112

Fig. 1.

Fig. 1.

Generation and characterization of PELP1 FBKO mice. (A) Schematic representation of endogenous (WT) and recombined locus mPELP1. (B) Confirmation of targeted clones by Southern blotting of genomic DNA. (C) Tail DNA PCR confirmation of bitransgenic PELP1 FBKO mice expressing CaMKIIα-Cre and loxpPELP1. (D) Confirmation of excision of floxed region in hippocampus and cortex of FLOX control and PELP1 FBKO mice using genomic PCR. (E) Immunohistochemical analysis of coronal sections of hippocampus, cortex, and hindbrain of control and PELP1 FBKO mice. (F) Lysates of hippocampus, cortex and cerebellum collected from control (PELP1 loxp/loxp), heterozygous (PELP1 loxp/−, CaMKIIαCre+/−), and homozygous PELP1 FBKO (PELP1 loxp/loxp, CaMKIIα-Cre+/−) mice were subjected to Western blotting with PELP1 antibody. β-actin was used as a loading control. (G) The data were quantified using Image J software and normalized to β-actin. C, Cre; F, FLOX. Bar graphs represent mean ± SEM. **P < 0.01.