Fig. 3.

E2-mediated enhancement of p-GSK3β and reduction of p-β-catenin is significantly attenuated in PELP1 FBKO mice. (A–D) Hippocampal lysates obtained from sham, sham E2, and at 10 min, 30 min, and 3 h after GCI/reperfusion from placebo and E2 treated ovariectomized female FLOX control mice (A and C) and PELP1 FBKO mice (B and D) were subjected to Western blotting to detect changes in the levels of p-GSK3β and p-β-catenin. GAPDH was used as an internal control. Representative blots show the attenuation of E2-mediated effects in PELP1 FBKO mice. Pla = placebo; R = reperfusion (C and D). Bar graphs represent mean ± SEM. *P < 0.05; **P < 0.01 versus sham. n = 4–5 mice per group.