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. 2015 Nov 16;112(48):E6673–E6682. doi: 10.1073/pnas.1516729112

Fig. S9.

Fig. S9.

Antisense oligonucleotide knockdown of PELP1 in the ovariectomized rat reverses E2-mediated neuroprotection after global cerebral ischemia. (A) Representative photomicrographs depict hippocampal CA1 neurons immunopositive for the neuronal nuclear marker NeuN 7 d after global cerebral ischemia. The experimental design is described in Fig. S2. PELP1 knockdown efficiency is also shown in Fig. S2. Note that E2-treated PELP1 AS knockdown rats had significantly fewer surviving NeuN-positive neurons compared with E2-treated MS animals after GCI, indicating a loss of E2 neuroprotection after PELP1 knockdown. Quantitative summary of data (means ± SE; n = 5–7 per group) is expressed as a percentage of NeuN-positive neurons per 250-μm medial CA1 of E2+MS and E2+AS animals. ^ = P < 0.05 compared with E2+MS. Magnification 40×. (Scale bar, 50 μm.) (B) Representative cresyl violet staining in the hippocampal CA1 region 7 d after global cerebral ischemia. Quantitative summary of data (means ± SE; n = 5–7 per group) is expressed as a percentage of cresyl violet-positive neurons per 250-μm medial CA1 of E2+MS and E2+AS animals. ^ = P < 0.05 compared with E2+MS. Magnification 40×. (Scale bar, 50 μm.)