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. 2015 Nov 16;112(48):E6673–E6682. doi: 10.1073/pnas.1516729112

Fig. S11.

Fig. S11.

Antisense oligonucleotide knockdown of PELP1 in the ovariectomized rat reverses E2 enhancement of cognitive function after GCI. (A) Latency trail and (B) probe trail in the Morris water maze. Representative tracks for latency trail (C) and probe trail (D), [a and f: sham; b and g: ischemia/reperfusion (I/R); c and h: E2; d and i: E2+MS; e and j: E2+PELP1-AS]. Note that E2-treated animals had significantly reduced latency times to find the hidden platform in the water maze (A and C) and spent more time in the quadrant where the platform was located compared with I/R animals (B and D). MS oligos had no effect on E2 enhancement of cognitive function. PELP1 AS oligo knockdown markedly attenuated E2’s effect to decrease latency to find the platform (A and C) and increase time spent in the quadrant where the platform was located (B and D). The experimental design is as described in Fig. S2. PELP1 AS knockdown efficiency is also shown in Fig. S2. *P < 0.05 versus I/R group, #P < 0.05 versus E2+MS or E2+AS, n = 5–6.