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Activated D570A persistently binds to DNA. (A) Equal amounts of purified phospho-STAT3 and D570A were incubated for 15 min with 32P-labeled m67SIE probe. Excess of cold probe was then added at time 0, and the protein–DNA complexes were loaded onto the running gel at the indicated times. (B) Quantification of the data shown in A.
