Fig. S1.

Tradeoffs in repression and expression over longer sequence space. (A) Valley depth in both environments for all direct 720 trajectories. Valley depth is the largest decrease along a mutational trajectory. (B) Valley width in both environments for all direct 720 trajectories. Valley width equals the number of mutations required to achieve a level that is greater than or equal to the previous suboptimum. Points of corresponding trajectories are depicted with a small random offset for visualization purposes. Selectively accessible trajectories are indicated in black. One does not observe selectively accessible trajectories that have a small (A) valley depth or (B) valley width in both repression and expression abilities.