Fig. S2.

Structure-based sequence alignment of the C7 family of Poxvirus host-range factors. The alignment was performed with the SSM server (19) by using the crystal structures of VACV-C7 and MYXV-M64 as the templates, and the figure was created with ESPript (38). VACV-C7 and MYXV-M64 secondary structures and residue numbers are denoted above and below the alignment, respectively. Colored asterisks indicate key residues on three distinct loops that were found to be critical for the function of VACV-C7 (red: acidic loop; blue: basic loop; magenta: hydrophobic loop).