Fig. S5.

Coimmunoprecipitation studies of additional C7 mutants. HeLa cells in a six-well plate were infected with VACV expressing the indicated C7 mutants at an MOI of 5 pfu per cell. At 8 hpi, the cells were lysed. V5-tagged C7 proteins were immunoprecipitated from the cell lysates with agarose conjugated with a monoclonal V5 antibody. The levels of SAMD9 and V5-tagged viral proteins in the cell lysates and precipitates were determined by Western blot with antibody against SAMD9 and V5, respectively. The mutants were studied in groups in four separate experiments (A, B, C, and D), each with a WT control. The experiment was performed twice with similar results, and a representative result is shown. Ig LC, the light chain of the anti-V5 antibody.