. 2015 Nov 17;112(48):14858–14863. doi: 10.1073/pnas.1515354112

Table 1.

Summary of the C7 mutagenesis study

Name	Mutations	Protein level^*	Viral growth^†	SAMD9 precipitation^‡
WT	None	+++	+++	+++
m1	Δ(141-150)	+++	+++	+
m2	K24E	+++	−	−
m3	K40E, K41E, K43E	++	+++	+++
m4	D51K, E54R, D56K, E57K	+++	−	−
m5	W52A	+++	−	−
m6	K59E	+	−	+
m7	F64A	+++	+++	+++
m8	F79S, Y80S, Y81S	+++	−	−
m9	K93E, K94E, E96K	+++	+++	+
m10	D102K, D103K, E104K, E106K	++	−	+
m11	K123E, K125E, K127D	+++	+++	+
m12	D51K, E54R	+++	−	−
m13	D56K, E57K	+++	+++	+++
m14	E6K, D8K	+	−	−
m15	D26K, N27A	+++	−	−
m16	R31E, K33E	++	−	−
m17	D38K, Y39A	+++	+++	+++
m18	R45E, R49E	+	−	−
m19	Y68A	+++	+++	++
m20	K71E, K74E, D76K, D77K	+++	+++	+++
m21	E85K, H89A, Y91A	+	−	+
m22	Y99A	+++	+++	+++
m23	F108A, K109E, H110A	+++	−	−
m24	Y111A, Y112A, Y114A	+	−	−
m25	E128K, E129K, Y131A	+++	++	+
m26	Y135A, E137K	+	−	−
m27	D51K	+++	−	−
m28	E54R	+++	−	−
m29	W52I	+++	−	−
m30	D102K, D103K	+++	+++	+++
m31	E104K, E106K	+++	+++	+++

Relative C7 protein level in VERO cells as shown in Fig. S4 and quantified by band intensity, indicating the effect of the mutations on protein stability. +++, >50% of the WT level; ++, ∼50% of the WT level, +, <20% of the WT level.

^{^†}

Viral growth in HeLa cells as shown in Fig. S3. +++, WT level of viral titers at 12 and 48 hpi; ++, ∼1/100 of the WT titer at 48 hpi; −, no growth.

^{^‡}

Coimmunoprecipitation of SAMD9 as shown in Fig. 4 and Fig. S5 and quantified by band intensity. +++, >50% of WT level of SAMD9 precipitation; ++, ∼50% of the WT level; +, <20% of the WT level; −, no precipitation.