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. 2015 Nov 16;112(48):E6624–E6633. doi: 10.1073/pnas.1508543112

Fig. 6.

Fig. 6.

Dysregulation of DNA elongation observed in UV-irradiated, Rad51-depleted samples depends on Mre11 and Primpol. (A) Schematic of the experiments shown in B–D. (B) IdU track length distribution at 20 min in untreated U2OS cells transfected with siLuc, siRad51 (sequence 1), and Mre11 (sequence 1). (C) IdU track length distribution at 20 min post-UV irradiation of U2OS cells treated with Rad51 and Mre11 siRNA. (D) IdU track length distribution at 20 min post-UV irradiation in Rad51-depleted U2OS cells treated with mirin and Mre11 siRNA. (E) Schematic of the experiments shown in F–H. (F) IdU track length distribution at 60 min of untreated U2OS cells transfected with control, siLuc, siRad51 (sequence 1), and siPrimpol (sequence 1). (G) IdU track length distribution at 60 min post-UV irradiation in U2OS cells transfected with Rad51 or Primpol siRNAs, or with the combination of both. (H) IdU track length distribution at 60 min post-UV irradiation in Rad51-depleted U2OS cells treated with polη and Primpol siRNA. In B–D, the dotted line corresponds to the ldU track length average of untreated samples. Two independent experiments were analyzed, obtaining similar results.