Fig. 6.
Combinatorial inactivation of PAM-β′1 and MBON-γ4>γ1γ2 has an additive effect on memory decay. (A) Memory decay curves of flies bearing UAS-Kir2.1; Gal80ts combined with the double Gal4 driver NP2397; R76B09 labeling both PAM-β′1 and MBON-γ4>γ1γ2. The memory retention of the +HS and −HS groups was compared at different time points after training. All statistically significant differences (t test) are marked. Data are means ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001; n ≥ 6. (B) Relationship between the observed effect and the predicted effect under the assumption of simple additivity. The performance index difference between the +HS and −HS groups (ΔPI) observed for combinatorial inactivation in A was plotted against what is expected from the summation of individual inactivation of PAM-β′1 (NP2397; SI Appendix, Fig. S10B) and MBON-γ4>γ1γ2 (R76B09; SI Appendix, Fig. S10C). The dashed line is a 1:1 relationship, and the solid line is a regression fit.