Table S2.
Essential genes in central carbon metabolism
| Synechococcus elongatus identification | Name | Pathway* | Essentiality | Conserved† | Redundant‡ |
| SynPCC7942_2297 | tal | Pentose phosphate pathway | Nonessential | 1 | 0 |
| SynPCC7942_0538 | tktA | Pentose phosphate pathway | Essential | 1 | 0 |
| SynPCC7942_0604 | cbbE | Pentose phosphate pathway | Essential | 1 | 0 |
| SynPCC7942_0584 | rpiA | Pentose phosphate pathway | Essential | 1 | 0 |
| SynPCC7942_0529 | pgl | Pentose phosphate pathway | Nonessential | 1 | 0 |
| SynPCC7942_2334 | zwf | Pentose phosphate pathway | Nonessential | 1 | 0 |
| SynPCC7942_0039 | gnd | Pentose phosphate pathway | Nonessential | 1 | 0 |
| SynPCC7942_0098 | pyk | Glycolysis | Essential | 1 | 0 |
| SynPCC7942_0639 | eno | Glycolysis | Essential | 1 | 0 |
| SynPCC7942_0469 | pgmM | Glycolysis | Essential | 1 | 1 |
| SynPCC7942_1116 | pgk | Glycolysis | Essential | 1 | 0 |
| SynPCC7942_1939 | gap3 | Glycolysis | Nonessential | 0 | 1 |
| SynPCC7942_0245 | gap1 | Glycolysis | Nonessential | 0 | 1 |
| SynPCC7942_1261 | tpiA | Glycolysis | Essential | 1 | 0 |
| SynPCC7942_1443 | cbbA | Glycolysis | Essential | 1 | 0 |
| SynPCC7942_0592 | pfkA | Glycolysis | Nonessential | 0 | 0 |
| SynPCC7942_2029 | pgi | Glycolysis | Essential | 1 | 0 |
| SynPCC7942_0781 | ppsA | Glycolysis | Nonessential | 0 | 0 |
| SynPCC7942_2335 | fbp | Glycolysis | Nonessential | 0 | 1 |
| SynPCC7942_0505 | fbpI | Glycolysis | Essential | 1 | 1 |
| SynPCC7942_0485 | pgam1 | Glycolysis | Essential | 1 | 1 |
| SynPCC7942_1516 | pgam2 | Glycolysis | Essential | 0 | 1 |
| SynPCC7942_2078 | pgam3 | Glycolysis | Nonessential | 0 | 1 |
| SynPCC7942_0612 | gltA | TCA cycle | Essential | 1 | 0 |
| SynPCC7942_0903 | acnB | TCA cycle | Essential | 0 | 0 |
| SynPCC7942_1007 | fumC | TCA cycle | Nonessential | 1 | 0 |
| SynPCC7942_1719 | icd | TCA cycle | Essential | 0 | 0 |
| SynPCC7942_1533 | sdhB | TCA cycle | Nonessential | 0 | 0 |
| SynPCC7942_1427 | cbbS | Calvin–Benson–Bassham cycle | Essential | 1 | 0 |
| SynPCC7942_1426 | cbbL | Calvin–Benson–Bassham cycle | Essential | 1 | 0 |
| SynPCC7942_1116 | pgk | Calvin–Benson–Bassham cycle | Essential | 1 | 0 |
| SynPCC7942_1742 | gap2 | Calvin–Benson–Bassham cycle | Essential | 1 | 0 |
| SynPCC7942_1261 | tpiA | Calvin–Benson–Bassham cycle | Essential | 1 | 0 |
| SynPCC7942_1443 | cbbA | Calvin–Benson–Bassham cycle | Essential | 1 | 0 |
| SynPCC7942_2335 | fbp | Calvin–Benson–Bassham cycle | Nonessential | 0 | 1 |
| SynPCC7942_0538 | tktA | Calvin–Benson–Bassham cycle | Essential | 1 | 0 |
| SynPCC7942_0505 | fbpI | Calvin–Benson–Bassham cycle | Essential | 1 | 1 |
| SynPCC7942_0604 | cbbE | Calvin–Benson–Bassham cycle | Essential | 1 | 0 |
| SynPCC7942_0977 | prk | Calvin–Benson–Bassham cycle | Essential | 0 | 0 |
| SynPCC7942_0584 | rpiA | Calvin–Benson–Bassham cycle | Essential | 1 | 0 |