Table S3.
Essential genes for the photosynthetic lifestyle
| Synechococcus elongatus identification | Name | Description | Cyano conserved* |
| SynPCC7942_0027 | bioF | 8-Amino-7-oxononanoate synthase | No |
| SynPCC7942_0203 | ribD | 5-Amino-6-(5-phosphoribosylamino)uracil reductase… | Yes |
| SynPCC7942_0222 | Hypothetical protein | No | |
| SynPCC7942_0226 | Sec-independent protein translocase TatA | No | |
| SynPCC7942_0257 | Protein of unknown function DUF92, transmembrane | Yes | |
| SynPCC7942_0297 | ftsH | FtsH peptidase homolog, chloroplast; metallopeptidase | No |
| SynPCC7942_0320 | galE | UDP-galactose 4-epimerase | No |
| SynPCC7942_0322 | ycf44 | c-Type cytochrome biogenesis protein | Yes |
| SynPCC7942_0323 | ccdA | Cytochrome c biogenesis protein-like | Yes |
| SynPCC7942_0330 | atp1 | Hypothetical protein | Yes |
| SynPCC7942_0333 | atpG | F0F1 ATP synthase subunit B′ | Yes |
| SynPCC7942_0439 | chlM | Mg-protoporphyrin IX methyl transferase | Yes |
| SynPCC7942_0450 | nifU | Putative NifU-like protein | Yes |
| SynPCC7942_0485 | gpmB | Phosphoglycerate mutase | Yes |
| SynPCC7942_0492 | ribF | Bifunctional riboflavin kinase/FMN adenylyltransferase | Yes |
| SynPCC7942_0537 | fabF | 3-Oxoacyl-(acyl carrier protein) synthase II | Yes |
| SynPCC7942_0560 | ATPase | No | |
| SynPCC7942_0584 | rpiA | Ribose-5-phosphate isomerase A | Yes |
| SynPCC7942_0632 | rplJ | 50S ribosomal protein L10 | Yes |
| SynPCC7942_0684 | fabG | 3-oxoacyl-[acyl-carrier-protein] reductase | Yes |
| SynPCC7942_0694 | rps1 | 30S ribosomal protein S1 | Yes |
| SynPCC7942_0775 | Hypothetical protein | No | |
| SynPCC7942_0853 | dapL1 | l,l-diaminopimelate aminotransferase | No |
| SynPCC7942_0884 | EF-Tu | Elongation factor Tu | Yes |
| SynPCC7942_0894 | aroK | Shikimate kinase | Yes |
| SynPCC7942_0897 | minE | Cell division topological specificity factor MinE | Yes |
| SynPCC7942_0912 | dnaX | DNA polymerase III, τ-subunit | Yes |
| SynPCC7942_0928 | Outer envelope membrane protein | No | |
| SynPCC7942_0942 | ftsH | FtsH peptidase homolog, chloroplast, metallo peptidase | No |
| SynPCC7942_0959 | obgE | GTPase ObgE | Yes |
| SynPCC7942_0977 | prk | Phosphoribulokinase | No |
| SynPCC7942_0978 | petH | Ferredoxin-NADP oxidoreductase | Yes |
| SynPCC7942_0988 | ycf54 | Conserved hypothetical protein YCF54 | No |
| SynPCC7942_1002 | psaD | Photosystem I reaction center subunit II | Yes |
| SynPCC7942_1068 | pdhC | Branched-chain α-keto acid dehydrogenase subunit E2 | Yes |
| SynPCC7942_1083 | Probable glycosyltransferase | Yes | |
| SynPCC7942_1086 | hemE | Uroporphyrinogen decarboxylase | Yes |
| SynPCC7942_1198 | phdD | Dihydrolipoamide dehydrogenase | Yes |
| SynPCC7942_1232 | petC | Cytochrome b6-f complex iron-sulfur subunit | Yes |
| SynPCC7942_1274 | ycf37 | TPR repeat | Yes |
| SynPCC7942_1351 | HAD-superfamily hydrolase subfamily IA, variant 3 | No | |
| SynPCC7942_1359 | Coenzyme F420 hydrogenase | No | |
| SynPCC7942_1427 | rbcS | Ribulose 1,5-bisphosphate carboxylase small subunit | Yes |
| SynPCC7942_1457 | plsC | 1-Acyl-sn-glycerol-3-phosphate acyltransferase | Yes |
| SynPCC7942_1497 | Hypothetical protein | No | |
| SynPCC7942_1499 | petF | Ferredoxin (2Fe-2S) | No |
| SynPCC7942_1520 | rpsT | 30S ribosomal protein S20 | Yes |
| SynPCC7942_1590 | Hypothetical protein | No | |
| SynPCC7942_1673 | Hypothetical protein | Yes | |
| SynPCC7942_1734 | ftrC | Ferredoxin-thioredoxin reductase catalytic chain | No |
| SynPCC7942_1737 | sufD | Iron-regulated ABC transporter permease protein SufD | Yes |
| SynPCC7942_1830 | trxA | Thioredoxin | Yes |
| SynPCC7942_1883 | ycf53 | Conserved hypothetical protein YCF53 | No |
| SynPCC7942_1907 | acsF | Mg-protoporphyrin IX monomethyl ester (oxidative) cyclase | No |
| SynPCC7942_1959 | proS | Prolyl-tRNA synthetase | Yes |
| SynPCC7942_1983 | pds | ζ-Carotene desaturase/three-step phytoene desaturase | No |
| SynPCC7942_2017 | Hypothetical protein | Yes | |
| SynPCC7942_2062 | crtL | Lycopene cyclase (CrtL-type) | No |
| SynPCC7942_2084 | chlG | Bacteriochlorophyll/chlorophyll a synthase | Yes |
| SynPCC7942_2089 | Thioredoxin domain 2 | Yes | |
| SynPCC7942_2113 | kprS | Ribose-phosphate pyrophosphokinase | Yes |
| SynPCC7942_2117 | gatA | Aspartyl/glutamyl-tRNA amidotransferase subunit A | Yes |
| SynPCC7942_2136 | dapB | Dihydrodipicolinate reductase | Yes |
| SynPCC7942_2274 | chlD | Protoporphyrin IX magnesium-chelatase | Yes |
| SynPCC7942_2312 | murG | UDP-N-acetylglucosamine-N-acetylmuramyl-(pentapeptide)… | Yes |
| SynPCC7942_2330 | ctpA | C-terminal processing peptidase-2; serine peptidase | No |
| SynPCC7942_2359 | nha3 | Na+/H+ antiporter | No |
| SynPCC7942_2415 | lysS | Lysyl-tRNA synthetase | Yes |
| SynPCC7942_2503 | por | Protochlorophyllide oxidoreductase | Yes |
| SynPCC7942_2524 | tig | Trigger factor | Yes |
| SynPCC7942_2531 | tsf | Elongation factor Ts | Yes |
| SynPCC7942_2537 | clpPIII | ATP-dependent Clp protease proteolytic subunit | Yes |
| SynPCC7942_2538 | clpR | ATP-dependent Clp protease-like protein | Yes |
| SynPCC7942_2581 | petF | Ferredoxin (2Fe-2S) | Yes |
S. elongatus essential genes conserved in the green lineage (plants and green algae) but not in nonphotosynthetic organisms as determined using the greencut2 dataset (30). GTPase, guanosine triphosphate hydrolase.
*
Refers to conservation among all 13 of the cyanobacterial genomes studied in the work by Shi and Falkowski (26).