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. 2015 Nov 16;112(48):14829–14833. doi: 10.1073/pnas.1510409112

Fig. 1.

Fig. 1.

Molecular masses of NifBMa and NifBMt. (A) Coomassie blue-stained 4–15% SDS/PAGE (BioRad Mini-PROTEAN TGX SDS/PAGE) of NifBMa and NifBMt. Lanes from left to right: 10 μL of protein standards (BioRad Precision Plus Protein Kaleidoscope Standards), 2 μg of purified NifBMa, and 2 μg of purified NifBMt. (B) Determination of the native molecular masses of NifBMa and NifBMt by gel filtration. V0, void volume; Ve, elution volume. Protein standards (GE Healthcare Biosciences), shown in open triangles, are: ribonuclease A (13.7 kDa), carbonic anhydrase (29 kDa), and ovalbumin (43 kDa).