Fig. 1.
Rf6 restores the fertility of HL-CMS rice. (A) Schematic diagram showing the mapping and location of Rf6. Clone number and restriction sites are indicated. (B) Functional complementation analysis of Rf6 in rescuing HL-CMS sterility. Orf2 was transformed into the sterile line YTA (rf6rf6) by Agrobacterium tumefaciens-mediated methods. The anthers, 1% I2-KI–stained pollen grains, and seed set rate for YTA and the transgenic plants carrying Orf2 are shown. (Scale bars: 1 mm, 50 µm, and 500 mm in anthers, pollen grains, and seeds, respectively.)