Fig. 2.

The duplicated PPR motifs 3–5 in RF6 are crucial for restoring fertility. (A) Localization of Rf6 in mitochondria. Protoplasts transformed with 35S:GFP:nos (CK) and 35S:Rf6-GFP: nos (Rf6) constructs were analyzed using fluorescence microscopy. The dye Mito-Tracker Red was used as a mitochondrial marker. (Scale bars: 10 µm.) (B) Comparison of the amino acid sequences for the RF6 and rf6 proteins. Sequence alignment was performed using DNAMAN6.0 software. The PPR motifs are indicated with solid lines. SP: signal peptide. (C) Schematic diagrams showing wild-type Rf6 and engineered Rf6L constructs (Rf6L-1–Rf6L-9). The crosses involved the deletion of the corresponding PPR motifs. F: fertility; S: sterility.