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. 2015 Dec 8;10(12):e0144338. doi: 10.1371/journal.pone.0144338

Fig 2. Modest and retarded increase in C/EBPβ mRNA under differentiation-inducing conditions.

Fig 2

(A) Under conditions as shown in Fig 1A, the expression of C/EBPβ mRNA in THP-1 cells was monitored by qPCR using a C/EBPβ-specific primer pair in the central mRNA section, and GAPDH was used as a housekeeping gene control (mean±SD; n≥6, measured in duplicates). The level of C/EBPβ mRNA in unstimulated cells at 0 h was defined as one (dashed line). (B) Under conditions as described in Fig 1A, 1C and 1D, C/EBPβ mRNA expression was measured in monocytic cell lines and analysed as described in A. THP-1 + VitD3, MM-6 + PMA: mean±SD; n = 9 each (duplicates). THP-1 + PMA (5’): qRT-PCR using an additional C/EBPβ-specific primer pair in the 5’ section of the mRNA; mean±SD; n = 4 (duplicates).