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Washed platelets (2.5 x108 platelets/mL) pretreated or not with ABT-737 (10 μM) were lysed and caspase-3 activity was assessed by immunoblotting with A) an anti-caspase-3 antibody B) with an anti-gelsolin antibody and C) with anti-calpain 1 antibody. Results are representative of two or three independent experiments.
